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Nested-PCR of ITS1-5.8S rDNA gene of Leishmania DNA from human samples and P. sergenti sand fly. Lines 1–7: human samples; line 8–10: P. sergenti females. Reference strains: L. infantum (MHOM/TN/80/IPT1), L. major (MHOM/SU/73/5ASKH) and L. tropica (MHOM/SU/74/K27); N: Negative control; 1 kb weight molecular (WM) (Invitrogen).
