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. 2001 Jul 3;98(15):8774–8779. doi: 10.1073/pnas.151236098

Figure 6.

Figure 6

P6 also induces NF-κB activation via the same TLR2–TAK1-dependent NIK–IKKα/β–IκBα and MKK3/6–p38 MAP kinase signaling pathways. (A) P6, but not LOS and HMW, induces NF-κB activation. HeLa cells were stimulated with either NTHi or polymyxin B-pretreated NTHi, or HMW (1.0 μg/ml) or P6 (0.005–0.5 μg/ml). PB, polymyxin B. (B) Inhibition of NIK–IKKα/β–IκBα signaling pathway blocks P6-induced NF-κB activation. (C) Inhibition of MKK3/6–p38 MAP kinase pathway abrogates P6-induced NF-κB activation. (D) Overexpression of a dominant-negative mutant of either TAK1 or TLR2 inhibits P6-induced NF-κB activation, whereas overexpression of a wild type of TLR2 enhances P6-induced NF-κB activation. Values are the means ± SD; n = 3.