Table 5.

Previous reports on EGFR mutation test from circulating free DNA

Year	Authors	Subjects	DNA concentration	Mutation test	Detection rate
2006	Kimura H, et al. [16]	Asian	70 ng/mL (range, 0–1720 ng/mL)	SARMS	48.1% (13/27)
		Female : 37%
		Nonsmoker : N/A
		ADC : 85%
		ORR : 33%
2008	Maheswaran S, et al. [24]	Western	N/A	SARMS	39% (7/18)
		EGFR mutant patients
2009	He C, et al. [29]	Asian	N/A	Mutant-enriched PCR	49.3% (66/134)
		Female : 37%
		Nonsmoker : 53%
		ADC : 75%
2009	Bai H, et al. [28]	Asian	N/A	dHPLC	34.3% (79/230)
		Female : 46%
		Nonsmoker : 55%
		ADC : 74%
		ORR : 36% (37/102)
2009	Mack PC, et al. [26]	Western/Asian : 96/4%	2.3 ng/μL (range, 1–9 ng/μL )	SARMS	20% (10/49)
		Female : 56%
		Nonsmoker : 53%
		ADC : 67%
2009	Kuang Y, et al. [25]	Western	52.3 ng/μL (range, 10–163 ng/μL )	SARMS and WAVE/Surveyor	54% (29/54)
		Female : 81.5%
				Whole genome amplification
		Nonsmoker : N/A
		ADC : N/A
		ORR : 56%
2010	Brevet M, et al. [31]	Western	N/A	Mass spectrometry genotyping assay (Sequenom) and mutant-enriched PCR Whole genome amplification	23.2% (10/31)
		Female : 52%
		Nonsmoker : 45%
		ADC : 97%
2010	Jian G, et al. [27]	Asian	N/A	Taqman PCR	23.2% (13/56)
		Female : 46%
		Nonsmoker : 58%
		ADC : 78%
		ORR : 30%
2011	Jiang B, et al. [30]	Asian	Minimum 4 ng/μL (range, 11–66 ng/μL )	Mutant-enriched PCR	31% (18/58)
		Female : 31%
		Nonsmoker : 38%
		ADC : 72%
2011	Taniguchi K, et al. [32]	Asian	N/A	BEAMing	72.7% (32/44)
		EGFR mutant patients
This study	Kim HR, et al.	Asian	8.6 ng/μL	PNA-based PCR clamping	16.7% (10/60)
		Female : 65%
		Nonsmoker : 2%
		ADC : 88%
		ORR : 100%

Abbreviations: ADC adenocarcinoma, SARMS scorpion-amplified refractory mutation system, PCR polymerase chain reaction, dHPLC denaturing high-performance liquid chromatography, BEAMing beads, emulsion, amplification, and magnetics, PNA peptide nucleic acid.