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. Author manuscript; available in PMC: 2013 Aug 23.
Published in final edited form as: Cell Immunol. 2011 Jun 12;271(1):44–52. doi: 10.1016/j.cellimm.2011.06.001

Fig. 5.

Fig. 5

Lymphocyte GH in enriched cytoplasmic and nuclear fractions of mouse spleen cells. Subcellular fractions were prepared as described in the methods. After SDS-PAGE (8%) and transfer to PVDF membrane, Western blot analysis was performed using commercial Ab to GH (Santa Cruz) and bands visualized using a chemiluminescence substrate for HRP. Blots shown below were stripped and reprobed with specific Abs to actin (lanes 1,2,4,5) or PCNA (lanes 3,6). The approximate molecular weight for isoforms of GH are shown with arrows on the right. Key; Lane 1 (T cells, whole cell); lane 2 (T cells, cytoplasm), lane 3 (T cells, nucleus); lane 4 (B cells, whole cell); lane 5 (B cells, cytoplasm); lane 6 (B cells, nucleus). Asterisks (*) denote a significant difference (p<0.05) in the T cell nuclear fraction to the whole T cell fraction and between the B cell nuclear fraction to the T cell nuclear fraction. The results shown above are typical of an experiment repeated 5 times.