Fig. 7.

GH protein expression in mouse spleen cells treated with DEM. Cells were treated for 16 hrs with different doses of DEM after which whole cell extracts were prepared as described in the methods. After SDS-PAGE (8%) and transfer to PVDF membranes, Western blot analysis was performed using commercial Ab to GH (Santa Cruz) and bands visualized using a chemiluminence substrate for HRP. Blots were stripped and reprobed with specific Abs to actin. Asterisks (*) denote a significant difference (p<0.05) from control. The approximate molecular weights for isoforms of GH are shown with arrows on the right. The results shown are typical of an experiment repeated 3 times. Key: Lane 1 (nontreated control); lane 2 (DEM, 1400 µM); lane 3 (DEM, 700 µM); lane 4 (DEM, 350 µM); lane 5 (DEM, 175 µM); lane 6 (DEM, 87.5 µM).