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. 2013 Mar 15;19(10):755–764. doi: 10.1089/ten.tec.2012.0524

FIG. 1.

FIG. 1.

CRX expression in manually manufactured embryoid bodies (EBs) after 17 days in the differentiation medium. (A) Reverse transcription polymerase chain reaction (RT-PCR) analysis showing CRX expression in EBs. MEB: mixed-size EBs, nonsupplemented medium; MEB+: mixed-size EBs, medium supplemented with taurine and triiodothyronine (T3); EB+s: manually selected EBs <100 μm/200 cells; EB+m: manually selected EBs ∼200 μm/1000 cells; EB+l: manually selected EBs >400 μm/5000 cells. WERI-Rb1 cells served as a positive control. (B) RT-qPCR comparing quantitative CRX expression. Expression in MEB and MEB+cells compared with EBs supplemented with taurine and T3 and manually sorted by size. Values represent the mean±standard error of the mean (SEM) from three independent samples, each repeated in triplicate. Change in the gene expression calibrated to expression in human embryonic stem cells. (C) Representative images of size-restricted EBs, generated manually. (C1) Small EBs (<100 μm/200 cells); (C2) medium EBs (∼200 μm/1000 cells); (C3) large EBs (>400 μm/>5000 cells).