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. 2013 Mar 15;19(10):755–764. doi: 10.1089/ten.tec.2012.0524

FIG. 2.

FIG. 2.

CRX expression in EBs formed with the AggreWell plates after 17 days in the differentiation medium. (A) RT-PCR analysis showing CRX gene expression in EBs made from 200, 1000, but not 10,000 cells. +/−: with or without taurine and T3 supplements. (B) RT-qPCR comparing CRX expression in mixed-size EBs with expression in 200 and 1000 cell EBs, suggesting a significant increase in expression in size-controlled EBs if supplements are added. +/−: with or without taurine and T3 supplements. Values represent the mean±SEM from three independent samples, each repeated in triplicate. Change in gene expression calibrated to expression in human embryonic stem cells. (C) Representative images of AggreWell EBs. (C1) 100 μm/200 cells; (C2) 200 μm/1000 cells; (C3) 300 μm/10,000 cells.