FIG. 3.

CRX protein levels in photoreceptor precursor cells (PPCs). (A) Western immunoblot analysis demonstrating CRX in cell lysates. MEB: mixed-size EBs compared with 200 cell and 1000 cell AggreWell EBs.+indicates the medium supplemented with taurine and T3. (B) Comparative flow cytometry analysis showing a highest proportion of CRX+ve cells in 1000-cell AggreWell EBs. Values represent the mean±SEM (n=6). (C) Representative dot plots and histograms from one flow cytometry experiment. Elliptical gate identifies CRX+ve cells. Color images available online at www.liebertpub.com/tec