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. 2013 Jul;11(6):367–381. doi: 10.1089/adt.2013.507

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Copyright 2013, Mary Ann Liebert, Inc.

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Fig. 5. — The specificity of the 14-3-3/pS136-Bad TR-FRET assay. (A) His-14-3-3ζ, but not mutant His-14-3-3ζ K49E, interacts with Dy647-pS136-Bad as measured by the TR-FRET assay. Increasing concentrations of His-14-3-3ζ, or His-14-3-3ζ K49E protein, was incubated with the Dy647-pS136-Bad peptide (20 nM) and anti–His-Eu (1 nM), and the TR-FRET signal was recorded. (B) The effect of unlabeled pS136-Bad peptide and unphosphorylated Bad peptide on the TR-FRET signal generated from the interaction of 14-3-3ζ with the Dy647-pS136-Bad peptide.