Fig. 5.

Temporal course of IAA concentrations in the lowest leaf (L6, a) and the basal stem (0.5 cm, b) of cuttings of P. hybrida during rooting under non-treated (solid line) and NPA-treated (dashed line) conditions (n = 5, each sample from two individual cuttings). Asterisks indicate a significant effect of the NPA treatment at the specified time after excision of cuttings (Mann–Whitney U test, P ≤ 0.05). c Transcript accumulation of auxin-responsive GH3 gene in the basal stem of P. hybrida during rooting under standard (non-treated) conditions. Northern blot analysis was performed with 20 g RNA per sample, and separation on a 1.5 % (w/v) formaldehyde agarose gel. After transfer of RNA to a nitrocellulose membrane, it was hybridized to radioactively labelled cDNA fragments of the corresponding gene. The below picture shows the loading control of rRNA, the ratio of intensity of 28S RNA to 18S RNA (for total RNA) is 1:1