Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jul 5;9:133. doi: 10.1186/1746-6148-9-133

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013 Cortes et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Osteogenic differentiation of bMSC isolated from fetal bone marrow. (A) Complex cell-interactions and extracellular matrix formation were observed in differentiating bMSC during a 27-Day culture period. (B) Higher (P < 0.05) levels of OC mRNA and ALPL activity were detected in differentiated bMSC at Days 16, 24 and 27 compared to untreated controls. NANOG mRNA levels increased (P < 0.05) from days 16 to 27 in differentiated mMSC. (C)CD73 mRNA expression was upregulated in untreated bMSC at Day 24 of osteogenic culture. (D) Activity of ALPL were higher (P < 0.05) in differentiated bMSC compared to control. (E) Osteogenesis was confirmed at Day 27 of culture by immunodetection of OC and (F) von Kossa staining of the matrix. Phase contrast (PC), OC and merged with DAPI for control and differentiation bMSC. Data are shown as mean ± standard errors. Superscripts (a,b,*) represent significant (P < 0.05) differences between treatment and sampling days. Bar scale: (A) 500 μm; (E) 20 μm; (F) 500 μm.