Table 1.
Gene | Sense | Antisense | Accession number |
---|---|---|---|
GAPDH |
5′ CCTTCATTGACCTTCACTACATGGTCTA |
5′ TGGAAGATGGTGATGGCCTTTCCATTG |
NM 001034034.2 |
βACTIN |
5′CGCACCACTGGCATTGTCAT |
5′TCCAAGGCGACGTAGCAGAG |
K00622.1 |
CD34 |
5′TGGGCATCGAGGACATCTCT |
5′GATCAAGATGGCCAGCAGGAT |
AB021662 |
CD45 |
5′CCTGGACACCACCTCAAAGCT |
5′TCCGTCCTGGGTTTTATCCTG |
NM 001206523 |
CD73 |
5′TGGTCCAGGCCTATGCTTTTG |
5′GGGATGCTGCTGTTGAGAAGAA |
BC114093 |
CD90 |
5′CAGAATACAGCTCCCGAACCAA |
CACGTGTAGATCCCCTCATCCTT |
BC104530 |
CD105 |
5′CGGACAGTGACCGTGAAGTTG |
5′TGTTGTGGTTGGCCTCGATTA |
NM 001076397 |
OC |
5′ TGACAGACACACCATGAGAACCC |
5′ AGCTCTAGACTGGGCCGTAGAAG |
EF673278.1 |
CD73 |
5′AATGGCACGATTACCTGGGA |
5′GGGAGGATCACCTTGTACAC |
BT026240.1 |
ACAN |
5′ CACTGTTACCGCCACTTCCC |
5′ GACATCGTTCCACTCGCCCT |
NM 173981.2 |
COL2A1 |
5′ ATCCATTGCAAACCCAAAGG |
5′ GACATCGTTCCACTCGCCCT |
NM 001113224.1 |
SOX9 |
5′CATGAAGATGACCGACGAG |
5′ CGTCTTCTCCGTGTCGGA |
AF278703.1 |
AP2 |
5′ CTGGCATGGCCAAACCCA |
5′ GTACTTGTACCAGAGCACC |
NM 174314.2 |
PPARγ |
5′ CGCACTGGAATTAGATGACAGC |
5′ CACAATCTGTCTGAGGTCTGTC |
BC116098.1 |
NANOG |
5′ GTGTTTGGTGAACTCTCCTG |
5′ GGGAATTGAAATACTTGACAG |
NM 001025344.1 |
OCT4 (1) |
5′ ACACTCGGACCACGTCTTTC |
5′ CGCATGGGTACCAGTGCACCTT |
AF022987 |
OCT4 (2) | 5′ GTTCTCTTTGGAAAGGTGTTC | 5′ ACACTCGGACCACGTCTTTC | AY490804.1 |
(1) and (2) represents sets of primers used for OCT4 mRNA amplification.