Table 2.
Unique amino acid changes in American genotype DENV2 (parental strains QR94 and PR159, PR159-derived midgut escape isolate EM41 and QR94-derived midgut escape isolate EM33) based on BLAST searches in NCBI GenBank
| Virus | C | prM | E | NS1 | NS2a | NS2b | NS3 | NS4a | NS4b | NS5 |
|---|---|---|---|---|---|---|---|---|---|---|
| QR94 |
|
E89G |
T55S |
I247Y |
T25S |
|
A373T |
|
|
N174D |
| L247Y |
K469R |
|||||||||
| F247Y | ||||||||||
| EM33 |
|
E89G |
T55S |
I247Y |
T25S |
|
A373T |
|
|
N174D |
|
E202K |
L247Y |
K469R |
||||||||
| F247Y | ||||||||||
| PR159 |
|
|
E202K |
M123T |
A88T |
|
|
|
|
S631N |
| EM41 |
Q77E |
M123T | A88T | E93D | S631N | |||||
| E202K |
In bold: additional amino acid substitutions identified in the midgut escape isolates EM33 and EM41, which were absent in the parental viruses.