Table 1.

Hybrid production between B. juncea, B. rapa, and B. kaber: frequency of embryo regeneration and hybrid plant establishment via in vitro embryo rescue

Cross combination	# of buds pollinated	# of siliques cultured	# of embryos excised	# of embryos germinated	# of hybrids obtained
B. juncea × B. kaber	100–150	80 (45 on A and 35 on B)	40 (22 on A and 18 on B)	6 (3 from medium A and 3 from medium B)	6
B. rapa × B. kaber	80–100	70 (40 on A and 30 on B)	50 (30 on A and 20 on B)	32 (18 from medium A and 14 from medium B)	32

A MS salts + Gamborg vitamins + 30 g/l sucrose + 500 mg/l casein hydrolysate (pH 5.8 and 8 g/l agar), B MS salts + Gamborg vitamins + 30 g/l sucrose + 0.5 mg/l NAA + 2.5 mg/l kinetin (pH 5.8 and 8 g/l agar)