FIG. 7.

Isoquercitrin, a major active compound of the AHB that was isolated from the AHE, induces apoptosis in HCT116 cells. (A) Chemical structures of the compounds that were isolated from the AHB. (B) HCT116 cells were treated with isoquercitrin (15, 30, and 60 μM) for 48 h. Cellular morphology was observed using a phase contrast microscope (above) and the sub G0/G1 content (%) was evaluated via flow cytometric DNA content analysis using the PI labeling method (below; bar=20 μm). (C) Schematic diagram illustrating the elucidation of the bifunctional chemopreventive effects of an A. himalaicum extract and the compounds that were isolated from it.