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. 2013 Aug 13;32(1):52. doi: 10.1186/1756-9966-32-52

Figure 2.

Figure 2

Downregulation of RABEX-5 in MCF-7 cell and effects of RABEX-5 on the colony formation and cell proliferation of breast cancer cells. (A), RABEX-5 mRNA levels were analyzed by real time-PCR. MCF-7 cells were transfected with pMAGic-siR lentiviral plasmid (MCF-7/KD) and pMAGic-siR-neg lentiviral control plasmid (MCF-7/NC). (B), RABEX-5 protein levels in MCF-7/KD and MCF-7/NC were analyzed by western blot. GAPDH was used as an internal control. P<0.05 compared with normal control (MCF-7) or MCF-7/NC. (C), CCK-8 cell proliferation assay for vector- and RABEX-5-transfecetd MCF-7 cells, curves indicate a significant level of proliferation compared to controls(P <0.05). (D), Representative colony formation assay, the numbers of colonies in MCF-7/NC were set to 100%. Values are expressed as mean±SD from three experiments, and the asterisks indicate statistical significance compared to controls (P<0.05).