Figure 3.

Downregulation of RABEX-5 expression inhibits cell migration. (A), Wound healing assay with MCF-7/NC and MCF-7/KD cells. Microscopic observations were recorded 0 and 54 hours after scratching the cell surface. a representative image from three independent experiments is shown. (B), Transwell assay. Photographs represented the cells travelled through the micropore membrane and histogram showed the percentage of migrant cells. (C), MMP-9 mRNA expression was evaluated by real time-PCR. The asterisk indicates statistical significant difference(P<0.05). Original magnification, ×40.