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Cell-based assay for proquorum sensing CRP ligands. Expression of the lux genes in Vibrio cholerae requires transcriptional activation of lux promoter by the quorum sensing regulator HapR and the cAMP-CRP complex. The cAMP-CRP complex is also required for Vibrio to make HapR. cAMP, cyclic adenosine monophosphate; CRP, cAMP receptor protein.
