Fig. 4.

Pilot HTS for proquorum sensing CRP-ligands. (A) Representation of pilot HTS for light induction in strain C7258Δcya containing pBB1. The line indicates the 3×standard deviations cutoff value used as “hit” criteria. (B) Secondary assay. Cultures of C7258Δcya (■, ▲) and C7258ΔcyaΔcrp (□, △) containing pBB1 were grown to stationary phase in LB and 100 μL of each culture added to triplicate wells of 96-well microtiter plates containing 100 μM of cAMP (■, □) and the 2nd hit compound (▲, △). Plates were incubated at 30°C and light production measured at different times. HTS, high throughput screening.