FIG. 6.

Leptin (a, b) and glycerol (c, d) levels increased when cultured under dynamic conditions (b, d) when compared with static cultures (a, c). The trends from static cultures were followed under dynamic conditions. No differences were seen when comparing 1 and 6 month cultures; however, the peak level was at 3 months. *Indicates significance level of p<0.001 across time points. Note: Error bars are not included in dynamic cultures as all constructs in one group were maintained in the same spinner flask and therefore we cannot decouple each individual construct contribution from the groups.