FIG. 4.

(a) Stress–strain curves for the three scaffolds (S-1, S-2, and S-3). (b) Young's modulus and maximum strength (n=5). (c) Optical density (OD) as determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after 12 h for scaffolds and respective wells and (d) cell-seeding efficiency (%); ([OD value of cells in scaffold]×100)/total OD value, for the scaffolds. *p<0.05 and NS indicate a significant difference and nonsignificance, respectively. Fluorescence images of the surface and interior regions of scaffolds showing live (green) and dead (red) cells after 12 h in culture on (e) S-1, (f ) S-2, and (g) S-3 scaffolds. (h) Comparison of initial cell viability (12 h) for surface and interior regions of the scaffolds. The scale bar in the magnified images indicates 100 μm. *p<0.05 and NS indicate a significant difference and nonsignificance, respectively. Color images available online at www.liebertpub.com/tec