Fig. 11.

Electron micrographs of cytosol derived from enterocytes isolated from WT mouse sacs fed either no oleate (A) or 1 mM oleate bound to albumin (B). Enterocytes were exposed to either no oleate or albumin bound 1 mM oleate for 2 min and the cytosol harvested. The cytosol was placed on a glow discharged nickel grid and photographed at 20,000 X in a JEOL electron microscope (Methods). 3 vesicles (white arrows) are shown in A and 12 vesicles (6 identified by white arrows) are shown in B. Electron micrographs of cytosol derived from enterocytes isolated from caveolin-1 KO mouse sacs exposed to no oleate (C) or 1 mM oleate bound to albumin (D). No vesicles were identified either on no oleate or oleate feeding. The bar is 500 nm. The methods utilized are the same as in Fig. 10. 4 grids of each group were counted.