Fig. 7–11.

Thin sections of host cell cultures (RK-13) exposed to spores of Anncaliia algerae for one hour. Post exposure (PE) the cells were briefly immuno-fixed (glutaraldehyde and paraformaldehyde), incubated in inosine-diphosphatase (IDPase) reaction media, then processed for TEM and stained with only uranyl acetate. The deposition of electron-dense reaction product (RP) in both the host cell Golgi cisternae and the sporoplasm MIN by this enzyme histochemical reaction, demonstrates the presence of enzymes indicative of cis-Golgi in both the host cell and MIN. 7. A mammalian host cell containing dense RP in the cis-Golgi cisternae (arrows) and some light staining of associated ER. Note the lack of RP in the other cytoplasmic (HC) organelles and the nucleus (Nu). The presence of host cell cis-Golgi containing RP, serves as a positive control of the IDPase enzyme histochemical labeling. The transverse section of the cisternae, appear as a series of parallel structures. Bar = 1.4 μm. 8. Higher magnification of a second host cell, containing several areas of IDPase labeled Golgi. This section further illustrates the specificity of the histochemical reaction labeling the cis-Golgi (arrows). Note the host cytoplasm (HC) and nucleus (Nu) are free of RP. Bar = 1.2 μm. 9. An extruded extracellular sporoplasm containing RP in the MIN (arrows), this label demonstrates the presence of IDPase activity in the MIN, indicating cis-Golgi properties. Note the rest of the sporoplasm cytoplasm is free of RP. Bar = 1.4 μm. 10. Sporoplasm (S), intracellularly located in a host cell cytoplasm (HC). Note the presence of dense IDPase label (arrows) in the peripheral region of the sporoplasm. Bar = 1.7 μm. 11. Higher magnification of Fig. 10. The host cell cytoplasm (HC) contains an IDPase labeled Sporoplasm (S) illustrating the presence of the electron-dense RP. The thick coat of RP (arrows) encompasses the entire periphery of the sporoplasm at the interface with the host cell. Bar = 600 nm.