Basolateral release of MIP3α by rat uterine epithelial cells in culture. Polarized uterine epithelial cells, grown in F12K complete medium in NUNC cell culture inserts, were treated apically with either heat-killed (HK) E. coli (A) or heat-killed L. rhamnosus (B). Following a 24-h incubation, basolateral media were harvested and analyzed for the presence of MIP3α. Values are means ± standard errors for three wells per group. * and **, significantly different (P < 0.05 and P < 0.01, respectively) from control. For E. coli, n = 5; for L. rhamnosus, n = 3.