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. 2004 Apr;72(4):1874–1884. doi: 10.1128/IAI.72.4.1874-1884.2004

FIG. 7.

FIG. 7.

Western blot analysis of CCS from wild-type, mutant, and complemented H. ducreyi strains with LspA1-specific MAb 40A4 (A) and LspA2-specific MAb 1H9 (B). Lane 1, wild-type strain 35000; lane 2, lspB mutant 35000.88; lane 3, lspA1 lspB mutant 35000.188; lane 4, 35000.88(pCW177) (vector-only control); lane 5, 35000.88(pCW225); lane 6, lspA1 mutant 35000.1. lspA1 mutant 35000.1 expressed readily detectable levels of LspA2 (66) and was used as a control to detect LspA2 expression. The positions of molecular mass markers are indicated on the left.