TABLE 1.
Description and origins of Neisserial strains used in this studya
| Strain | Serogroup type:subtype | 2086 subfamily | Source | Location (country) | 2086 expression on Western blot | Phylogenetic cluster |
|---|---|---|---|---|---|---|
| M98 250670 | B:1:P1.4 | B | MPHL | UK | + | B8 |
| M98 250024 | B:1:P1.4 | B | MPHL | UK | + | B5 |
| M97 253524 | B:1:P1.4 | B | MPHL | UK | + | B8 |
| M97 252060 | B:1:P1.4 | B | MPHL | UK | + | B5 |
| M97 251870 | B:4z:P1.4 | B | MPHL | UK | + | B5 |
| M97 251854 | B:4z:P1.4 | A | MPHL | UK | + | A1 |
| M97 251836 | B:4z:P1.4 | B | MPHL | UK | + | B5 |
| M97 251830 | B:4z:P1.4 | B | MPHL | UK | + | B5 |
| M97 251905 | B:4z:P1.4 | B | MPHL | UK | + | B5 |
| M97 251898 | B:4z:P1.4 | B | MPHL | UK | + | B5 |
| M97 251885 | B:4z:P1.4 | B | MPHL | UK | + | B8 |
| M97 251876 | B:4z:P1.4 | B | MPHL | UK | + | B5 |
| M97 251994 | B:4z:P1.4 | B | MPHL | UK | + | B5 |
| M97 251985 | B:4z:P1.4 | B | MPHL | UK | + | B5 |
| M97 251957 | B:4z:P1.4 | B | MPHL | UK | + | B5 |
| M97 251926 | B:4z:P1.4 | B | MPHL | UK | + | B8 |
| M97 252045 | B:4z:P1.4 | B | MPHL | UK | + | ND |
| M97 252038 | B:4z:P1.4 | B | MPHL | UK | + | ND |
| M97 252026 | B:4z:P1.4 | B | MPHL | UK | + | ND |
| M97 252010 | B:4z:P1.4 | B | MPHL | UK | + | ND |
| M97 252098 | B:4z:P1.4 | B | MPHL | UK | + | ND |
| M97 252083 | B:4z:P1.4 | B | MPHL | UK | + | ND |
| M97 252078 | B:4z:P1.4 | B | MPHL | UK | + | ND |
| M98 250735 | B:4z:P1.15 | B | MPHL | UK | + | ND |
| M98 250797 | B:4z:P1.15 | B | MPHL | UK | + | ND |
| M98 250768 | B:4z:P1.15 | B | MPHL | UK | + | ND |
| M98 250622 | B:2b:P1.10 | A | MPHL | UK | + | A1 |
| M98 250572 | B:2b:P1.10 | A | MPHL | UK | + | A1 |
| M98 250716 | B:2b:P1.10 | A | MPHL | UK | + | A1 |
| M98 250699 | B:4z:P1.10 | B | MPHL | UK | + | ND |
| M98 250393 | B:4z:P1.10 | B | MPHL | UK | + | ND |
| M98 250173 | B:4z:P1.10 | B | MPHL | UK | + | ND |
| M98 250771 | B:4z:P1.22,14 | A | MPHL | UK | + | A12 |
| M98 250732 | B:4z:P1.22,14-1 | A | MPHL | UK | + | A12 |
| CDC-1343 | B:NT:P1.22,14 | B | CDC | US | + | B1 |
| CDC-2369 | B:NT:P1.22-1,14-1 | A | CDC | US | + | A7 |
| 6557 | B:7:P1.22-1,14 | A | RIVM | US | + | A3 |
| M97 253462 | B:4z:P1.14 | B | MPHL | UK | + | ND |
| CDC-1127 | B:NT:P1.7,16 | B | CDC | US | + | ND |
| CDC-982 | B4:P1.7,16 | B | CDC | US | + | ND |
| CDC-1359 | B4:P1.7,16 | B | CDC | US | + | ND |
| M98 250762 | B:15:P1.7,16 | B | MPHL | UK | + | ND |
| M98 250610 | B:15:P1.7,16 | B | MPHL | UK | + | ND |
| M98 250626 | B:15:P1.16 | B | MPHL | UK | + | ND |
| M98 250809 | B:15:P1.7,16 | A | MPHL | UK | + | A2 |
| M97 253248 | B:15:P1.7,16 | A | MPHL | UK | + | A2 |
| M97 252029 | B:15:P1.7,16 | B | MPHL | UK | + | ND |
| M97 251875 | B:15:P1.7,16-10 | B | MPHL | UK | + | ND |
| CDC-798 | B:15:P1.7,16 | B | CDC | US | + | ND |
| CDC-1078 | B:15:P1.7,16 | B | CDC | US | + | ND |
| CDC-1614 | B:15:P1.7,16 | B | CDC | US | + | ND |
| CDC-1658 | B:15:P1.7,16 | B | CDC | US | + | ND |
| CDC-937 | B:15:P1.7,16 | B | CDC | US | + | B4 |
| H44/76 | B:15:P1.7,16 | B | RIVM | Norway | + | B4 |
| M97 250571 | B:15:P1.16 | B | MPHL | UK | + | B4 |
| M97 252097 | B:15:P1.16 | B | MPHL | UK | + | B4 |
| M97 253092 | B:1:P1.6 | B | MPHL | UK | + | ND |
| M97 252697 | B:1:P1.18,25,6 | A | MPHL | UK | + | A3 |
| M97 252988 | B:4:P1.18,25,6 | A | MPHL | UK | + | A3 |
| M97 252976 | B:4:P1.18,25,6 | A | MPHL | UK | + | A1 |
| M97 252153 | B:4:P1.18,25,6 | A | MPHL | UK | + | A11 |
| 6940 | B:NT:P1.18,25,6 | B | RIVM | US | + | B2 |
| CDC-1610 | B:4:P1.18-7,16-4 | A | CDC | US | + | A3 |
| CDC-1521 | B:2b:P1.6 | A | CDC | US | + | A1 |
| CDC-1985 | B:4:P1.7,13 | B | CDC | US | + | ND |
| CDC-1034 | B:4:P1.7 | A | CDC | US | + | A5 |
| L6 M992 | B:NT:P1.7,1 | ND | Walter Reed | + | ND/PICK> | |
| L8 M978 | B:15:P1.7,1 | A | Walter Reed | + | A3 | |
| CDC-1492 | B:4:P1.7,1 | A | CDC | US | + | A3 |
| CDC-1573 | B:4:P1.7-1,1 | B | CDC | US | + | B9 |
| L7 6155 | B:4:P1.7,1 | B | Walter Reed | + | ND | |
| 8529 | B:15:P1.7-2,3 | B | RIVM | Chile | + | B4 |
| 880049 | B:4:P1.7-2,4 | B | RIVM | Netherlands | + | B8 |
| 870446 | B:4:P1.12-1,13 | A | RIVM | Netherlands | + | A2 |
| CDC-2367 | B:4:P1.15 | B | CDC | US | + | B4 |
| H355 | B:15:P1.19,15 | B | RIVM | Norway | + | B4 |
| M982 | B:9:P1.22,9 | B | RIVM | US | + | B6 |
| 870227 | B:4:P1.5-3,10 | B | RIVM | Netherlands | + | B4 |
| B40 | A:NT:P1.5-3,10 | B | RIVM | + | B5 | |
| 5315 | B:4:P1.5-3,10 | B | RIVM | + | B5 | |
| 2996 | B:2b:P1.5-1,2-2 | A | RIVM | US | + | A1 |
| NMB | B:2b:P1.5-1,2-2 | A | UIOWA | US | + | A1 |
| CDC-983 | B:2a:P1.5,2 | B | CDC | US | + | B3 |
| L3 6275 | B:2a:P1.5,2 | A | Walter Reed | + | A8 | |
| CDC-852 | B:NT:P1.5,2 | B | CDC | US | + | B7 |
| B16B6 | B:2a:P1.5,2 | A | RIVM | + | A4 | |
| L4 891 | C:NT:P1.21,16 | A | Walter Reed | + | A6 | |
| L5 M981 | B:4:P1.21-6,1 | A | Walter Reed | + | A1 | |
| CDC-1135 | B:NT | A | CDC | US | + | A9 |
| A4 | A:NT | B | + | B5 | ||
| C11 | C:16,P1.7-1,1 | A | CDC | Germany | + | A4 |
| Y | Y: | A | Walter Reed | + | A4 | |
| W135 | W135: | A | Walter Reed | + | A4 | |
| Ng-FA1090 | A | UNC | US | + | A10 | |
| Nl-UR5 | A | UR | + | ND | ||
| Ns-UR4 | — | UR | − | ND |
a
Strains are arbitrarily arranged by PorA serosubtype. The 2086 gene subfamily was determined by PCR screening as described in Materials and Methods. A dash indicates negative results; ND indicates not done. Country or origin of each neisserial isolate is listed where known. UK, United Kingdom; US, United States. The 2086 gene was sequenced from those strains where a phylogenetic cluster number based on amino acid sequence homology is indicated (see Fig. 1).