TABLE 2.

Primers used in PCR amplification experiments

Primer name	Sequence	Restriction site (underlined)
2086NDE	5′-CTATTCTGCATATGACTAGGAGC-3′	NdeI
3STP2086	5′-GCGCGGATCCTTACTGCTTGGCGGCAAGACC-3′	BamHI
5UNI2086	5′-CTATTCTGCGTATGACTAG-3′	NAa
3UNI2086	5′-GTCCGAACGGTAAATTATCGTG-3′	NA
5INT2086	5′-TGCCGATGCACTAACCGCACC-3′	NA
3INT2086	5′-CGTTTCGCAACCATCTTCCCG-3′	NA
5OUT2086	5′-GACAGCCTGATAAACC-3′	NA
3OUT2086	5′-GATGCCGATTTCGTGAAC-3′	NA
N2086ILE	5′-GCGCAGATCTCATATGAGCAGCGGAGGGGGTGGTGTCGCCGCCGAYATWGGTGCGGGGCTTGCCG-3′	BglII/NdeI
20863STP	5′-GAGATCTCACTCACTCATTACTGCTTGGCGGCAAGACCGATATG-3′	BglII
N1573PCR	5′-GAGATCTCATATGAGCAGCGGAGGCGGCGGAAGC-3′	BglII
N29963STP	5′-GAGATCTCACTCACTCACTACTGTTTGCCGGCGATGCCGATTTC-3′	BglII
5LIP2086	5′-GCGGATCCAGCGGAGGGGGTGGTGTCGCC-3′	BamHI
3SPH2086	5′-GCGCATGCTTACTGCTTGGCGGCAAGACCGATATG-3′	SphI
5LIP1573	5′-GCGGATCCAGCGGAGGCGGCGGAAGC-3′	BamHI
5LIP2996	5′-GCGGATCCAGCGGAGGCGGCGGTGTCGCC-3′	BamHI
N2996SPH	5′-GCGCATGCCTACTGTTTGCCGGCGATG-3′	SphI
5LIP771	5′-GCGGATCCAGCGGAAGCGGAAGCGGAGGCGGCGGTGTCGCC-3′	BamHI
PorABg12Fwd	5′-CGCGAGATCTCATATGGATGTCAGCCTATACGGCGAAATCAAAGCCGGCGTGGAAGGCAGGAACTACCAG-3′	BglII/NdeI
PorABg12Ter	5′-CGCGAGATCTTCAGTCACTCATTAGAATTTGTGGCGCAAACCGACG-3′	BglII

^aNA, not applicable.