TABLE 2.

Assessment of the interaction of P. gingivalis with murine inflammatory cells in murine chamber fluids by fluorescent microscopy^a

Group	Data for day 1		Data for day 3
	P. gingivalis/ PMNb	% Phagocytosisc	P. gingivalis/ PMN	% Phagocytosis
1	NDd	ND	ND	ND
2	4.81 ± 4.35	55.39 ± 42.29	3.90 ± 3.68	42.41 ± 20.45
3	0.24 ± 0.19	4.33 ± 4.91	5.62 ± 7.81	37.35 ± 27.93
4	8.08 ± 7.95	59.15 ± 30.45	2.82 ± 1.91	44.34 ± 17.41
5	7.12 ± 7.27	53.65 ± 39.66	5.66 ± 4.20	52.32 ± 14.85
6	14.81 ± 4.5	40.79 ± 44.08	1.53 ± 1.32	30.92 ± 21.94

^aGroups of mice (n = 8 animals per group) were challenged subcutaneously with P. gingivalis, and chamber fluid samples were harvested at 1 and 3 days after challenge. Group 1 received vehicle only, group 2 was challenged with P. gingivalis, group 3 received P. gingivalis-specific IgG injection into the chamber 24 h prior to challenge, group 4 received IRR-IgG injection prior to challenge, group 5 was challenged with P. gingivalis opsonized with P. gingivalis-specific IgG, and group 6 was challenged with IRR-IgG-opsonized P. gingivalis.

^bP. gingivalis/PMN was determined as the total number of live and dead bacteria present within at least 100 live PMNs.

^c% Phagocytosis was determined as the number of viable PMNs that have at least one associated bacterium divided by the total number of PMNs counted, all multiplied by 100.

^dND, not determined.