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. 2004 Apr;72(4):1906–1913. doi: 10.1128/IAI.72.4.1906-1913.2004

FIG. 5.

FIG. 5.

Western blot analysis of and adherence assays with E. coli recombinant bacteria. (A) Proteins present in whole-cell lysates of EPI300 pCC1.3 and EPI300 pMHCD1.2 were resolved by SDS-PAGE and then analyzed by Western blotting with the OMPCD-specific MAb 1D3. These lysates were prepared from cells grown in the presence of the Epicentre's CopyControl inducer solution for 2 h. Positions of molecular mass markers are shown to the right in kilodaltons. (B) Visual adherence assays. Recombinant bacteria were grown under the aforementioned conditions. Unbound bacteria were washed off A549 cells after a 5-min incubation, and the monolayers were stained with Giemsa. (C) Quantitative adherence assays. Results are expressed as the percentage (± standard error) of recombinant bacteria binding to A549 cells after 5 min of incubation.