Figure 2.

OVA-specific humoral immune responses in lung washes from newborn lambs gavaged with OVA then i.p. immunized with OVA at 4 weeks of age. Lambs (n = 4/group) were gavaged and i.p. immunized as described in Figure 1A. Control newborn lambs were not gavaged or immunized with OVA. Lung lavages were collected 3 weeks post i.p. immunization and OVA-specific serum IgA (A) and IgG titres (B) were measured. ELISA titres are expressed as the reciprocal of the highest dilution resulting in a reading of two standard deviations above the negative control. Each data point represents an individual animal and median values are indicated by horizontal lines. *p < 0.05, ***p < 0.001.