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. 2013 Aug 12;11:57. doi: 10.1186/1478-811X-11-57

Figure 2.

Figure 2

PTP1B regulates Munc18c tyrosine phosphorylation. A) Munc18c was immunoprecipitated from lysates of PTP1B KO adipocytes and KO adipocytes reconstituted with PTP1B WT and D/A at basal and insulin-stimulated conditions then immunoblotted using antibodies for phosphotyrosine and Munc18c. B) Munc18c was immunoprecipitated from subcutaneous adipose tissue of control (fl/fl) and adipose-specific PTP1B KO fed HFD then immunoblotted using antibodies for phosphotyrosine and Munc18c. C) Munc18c was immunoprecipitated from PTP1B KO adipocytes and KO adipocytes reconstituted with WT at basal and insulin-stimulated conditions then immunoblotted using antibodies for syntaxin4 and Munc18c. Bar graph represents normalized data presented as means ± SEM. (**; P ≤ 0.01) indicates significant difference between insulin treated and non-treated cells, and (##; P ≤ 0.01) indicates significant difference between WT and KO cells for the corresponding treatment.