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. Author manuscript; available in PMC: 2013 Aug 23.
Published in final edited form as: J Comp Neurol. 2012 Feb 15;520(3):606–619. doi: 10.1002/cne.22753

Figure 1.

Figure 1

RNA isolation and cDNA amplification from single inspiratory pre-BötC neurons. A: Outline of mouse medullary slice showing experimental setup. B: Typical inspiratory pre-BötC neuron identified by a burst of activity concurrent XII nerve output. C: Patch/intracellular RNA aspirating electrode on inspiratory neuron viewed under infrared illumination. D: cDNA amplification from single neurons. Each lane represents cDNA enriched from a single pre-BötC inspiratory or NA neuron. NA, nucleus ambiguus; pre-BötC, pre-Bötzinger complex; XII, hypoglossal nucleus; XIIn, hypoglossal nerve. Scale bar = 10 µm.