Table 1 (panels A, B).
Number of identified proteins | Number of identified peptides | Number of identified spectra | |
---|---|---|---|
Single phase-LTQ | 628 | 3,316 | 6,880 |
MudPIT-Orbitrap-CID | 1,263 | 14,846 | 102,314 |
Directed MS/MS (TCA-DDA)a | 932 | 6,085 | 9,880 |
Directed MS/MS (acetone)a | 624 | 3,343 | 5,433 |
aDetailed results of directed MS/MS combining the two protein precipitation approaches are listed in Table S2 and Figure S7.. |
Panel B: Average numbers of identified Pyrococcus furiosus proteins, peptides, and spectra in three categories of experimental setups and instruments. In each case the results show averages and standard deviations (in parentheses) over three replicate experiments. False discovery rates are estimated to be below 0.5%. | |||
Number of identified proteins | Number of identified peptides | Number of identified spectra | |
MudPIT-LTQ | 1,279 (9) | 11,004 (522) | 105,215 (12,210) |
MudPIT-Orbitrap-ETD | 1,015 (23) | 7,548 (1,611) | 52,992 (10,370) |
QTOF-fractionation | 1,024 (47) | 9,328 (962) | 35,492 (7,205) |