Table 2 (panels A, B).
Panel A: Comparison of the percentage of shared peptides observed in a P. furiosus experiment and an S.cerevisiae experiment. Shared peptides are defined as identified peptides whose amino acid sequence is shared by two or more proteins in the database. The P. furiosus data set shows a level of redundancy that is an order of magnitude less than that of S. cerevisiae (0.9% vs. 10.4% shared peptides).
Organism | Identified peptides | Unique peptides | Shared peptides | Percent shared |
---|---|---|---|---|
P. furiosus | 12,251 | 12,136 | 115 | 0.9% |
S. cerevisiae | 7,786 | 6,977 | 809 | 10.4% |
Panel B: Results of a P. furiosus proteomics experiment analyzed with a concatenated protein database formed by adding a decoy organism protein database to that of P. furiosus. Regardless of the decoy protein database added we identify 1,260 unique P. furiosus proteins and 4 redundant P. furiosus proteins. In addition a very small number of identified peptides (1 to 3, depending on the decoy database) have amino acid sequences shared with decoy organism proteins. | |||
Decoy Organism | Unique P. furiosus peptides | Redundant P. furiosus peptides | Decoy organism proteins |
S. cerevisiae | 1,260 | 4 | 3 |
E. coil | 1,260 | 4 | 1 |
H. sapiens | 1,260 | 4 | 1 |