Table 1.
Replication of the suppressor virus and the γ34.5 mutant virus in human cancer cells
Virus | Titer of virus grown in PC3 cells | Titer of virus grown in U373 cells |
---|---|---|
WT | 1.1 × 108 | 9 × 108 |
SUP | 3 × 107 | 8.1 × 107 |
Δ34.5 | 2.8 × 105 | 4.3 × 103 |
PC3 or U373 (3 × 106) cells were infected with 103 pfu of either the γ34.5 deletion mutant Δ34.5, the SUP suppressor virus, or wild-type HSV-1 (WT). After 5 days, cell-free lysates were prepared and titered in permissive African Green Monkey kidney (Vero) cells.