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. 2013 Jul 30;20(1):52. doi: 10.1186/1423-0127-20-52

Figure 4.

Figure 4

Methylation-specific PCR analysis for NDRG1 methylation in MDA-MB-231 (MDA) and T47D breast cancer cell lines (A), breast cancer tissues (T) and corresponding normal breast tissues (N) (B). Genomic DNA from breast cancer cell lines and breast tumors treated with sodium bisulfite were amplified using methylated (M) and unmethylated primers (U). MC was used as a positive control for methylated reaction, and UC was used as a positive control for unmethylated reaction. NTC was blank control.