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. 2013 Aug 8;9:154. doi: 10.1186/1746-6148-9-154

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Copyright © 2013 Mtshali and Mtshali; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Phylogenetic tree based on rap-1 gene sequences (947 nt) of B. bovis isolates identified in this study (indicated in bold) and those of strains whose sequences were extracted from GenBank (accession numbers in parentheses). The tree was constructed using the neighbour-joining method, with bootstrap values (expressed as percentages of 1000 replications) superimposed at branching points; only the values above 60% are shown. The horizontal bar represents the number of base substitutions per site. Isolate designations: MP - Mpumalanga province, NW - North West province, WC - Western Cape province, and KZN - KwaZulu-Natal province.