Table 1.

Primary and nested PCR primers used for PCR amplifications

Species	Assay	Primer sequence (5′ → 3′)	Annealing	Product size
Oligonucleotides used for screening (Group I)a
B. bigemina	PCR	F-CATCTAATTTCTCTCCATACCCCTCC	55°C	278 bp
		R-CCTCGGCTTCAACTCTGATGCCAAAG
	nPCR	F-CGCAAGCCCAGCACGCCCCGGTGC	55°C	170 bp
		R-CCGACCTGGATAGGCTGTGTGATG
B. bovis	PCR	F-CACGAGGAAGGAACTACCGATGTTGA	55°C	360 bp
		R-CCAAGGAGCTTCAACGTACGAGGTCA
	nPCR	F-TCAACAAGGTACTCTATATGGCTACC	55°C	298 bp
		R-CTACCGAGCAGAACCTTCTTCACCAT
Oligonucleotides used for phylogenetic study (Group II)b
B. bigemina	PCR	F-GTGCTGCTTAATCGCACAAAC	55°C	963 bp
		R-AAGATGCCTTCTTCGGTGATG
	nPCR	F-CGGATCCTGTTATCGTTCCTG	56°C	853 bp
		R-GAAGTTACGCCTGGAGTTGG
B. bovis	PCR	F-TCAGATTGTTCAAAGAGAGTGCATCC	55°C	1280 bp
		R-GTCTTCACCGTTGGAAGTAGTTGAGTC
	nPCR	F-CACGAGGAAGGAACTACCGATGTTGA	64°C	1009 bp
		R-CCTTTGTAGGTTGGCCAACAGTTTCG

^a Group I oligonucleotide sequences were taken from published work [8].

^b Group II primers were designed in this study.