FIG. 3.
Effects of A. actinomycetemcomitans LPS and/or CHX on cytochrome c release, expression of Bcl-2 family proteins and phosphorylation of Akt and Bad. TPA-differentiated U937 cells (107 cells [A] or 106 cells [B and C]) were treated with A. actinomycetemcomitans LPS (1 μg/ml) in the presence or absence of CHX (10 μg/ml) for 15 min (C) or 3 h (A and B). Following each treatment, cytochrome c release (A); expression of Bcl-2, Bcl-xL, Bax, and Bak proteins (B); and phosphorylation of Bad (Ser136, Ser112) and Akt (Thr308) (C) were evaluated by Western blot analysis, as described in Materials and Methods.