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Quantitative PCR confirmed the METH-induced changes in gene expression. Acute METH administration increased the expression of (A) DnajB5, (B) Egr1, (D) Nptx2, and (E) Nts, but not (C) Npb in METH-naïve rats. In contrast, the acute METH injection caused increases in the expression of (C) Npb and (E) Nts in the METH-pretreated rats, with the effects of acute METH on Nts expression being significantly attenuated in the METH-pretreated rats.
