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. 2013 Aug 15;3:45. doi: 10.1186/2191-0855-3-45

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Copyright ©2013 Tomisawa et al.; licensee Springer.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression and purification of recombinant ABF-2. Lane 1: Inclusion body after ultrasonication and centrifugation. Lane 2: Solubilized inclusion body. Lane 3: A flowthrough fraction that was passed through cation-exchange chromatography. Lane 4: Purified ABF-2 using cation-exchange chromatography. Lane 5: Purified correctly folded ABF-2 using RP-HPLC.