TABLE 1.
Invasion of KB and HUVEC cells and attachment to HUVEC cells by different fusobacteria
| Strain | Invasiona (%)
|
Attachment to HUVEC cells (%) | |
|---|---|---|---|
| KB cells | HUVEC cells | ||
| F. nucleatum 12230 | 1.5 ± 0.0 | 0.5 ± 0.1 | 1.5 ± 0.6 |
| F. nucleatum 12230 lam | 0.4 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 |
| F. nucleatum DUMC 2929 | 1.5 ± 0.1 | 0.2 ± 0.0 | 0.5 ± 0.0 |
| F. nucleatum DUMC 3349 | 2.2 ± 0.5 | 2.0 ± 0.3 | ND |
| F. nucleatum DUMC 1356 | 2.3 ± 0.2 | 0.6 ± 0.1 | ND |
| F. nucleatum DUMC 2079 | 1.3 ± 0.1 | 0.4 ± 0.1 | 1.5 ± 0.6 |
| F. gonidiaformans DUMC CF65-1 | <0.01 | <0.01 | <0.01 |
| F. gonidiaformans DUMC CF63-1 | <0.01 | <0.01 | <0.01 |
| E. coli DH5α | <0.01 | <0.01 | ND |
a
Invasion and attachment were calculated as (no. of bacteria recovered after cell lysis) × 100/(total bacteria initially added). The values here are results from one representative experiment, shown as the average of duplicates ± standard deviation. Each experiment was repeated at least three times. The limit of detection was <0.01%. ND, not determined.