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. 2013 Aug 19;13:66. doi: 10.1186/1472-6750-13-66

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Copyright © 2013 Mathot et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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A workflow for serial extraction of DNA and RNA in tissue biobanks. Tumor and patient-matched normal biopsies and surgical specimens are embedded in OCT compound and frozen. Next, tissue sections are sectioned and stained for inspection by a pathologist and subsequent sections put in barcoded tubes in a tube rack for extraction. When a full rack of specimens has been generated, DNA and RNA molecules are serially extracted using the process presented in this work. The resulting nucleic acid fractions are quantitated and packaged in tube racks of sortable 96- or 384-well tubes for storage in automated freezers.