Table 1.
List and description of NP-like genes within Populus trichocarpa used for quantitative gene expression analyses and qPCR primer information
| Gene symbol | Gene name | Locus name Phytozome v2.2 (POPTR) and v3.0 (Potri) | Primers (5′-3′) | Product size (bp) | Annealing temp (°C) | PCR efficiency |
|---|---|---|---|---|---|---|
|
BSP A |
Bark Storage Protein A |
POPTR_0013s10380 |
F: TGGAGAGAACTTGTTGGGGAC |
81 |
55 |
1.981 |
| Potri.013G100700 |
R: CAGAAAACTTCCTTGGGCG |
|||||
|
BSP B |
Bark Storage Protein B |
POPTR_0013s10370 |
F: ATGTTCTCTCCAAGTGAAGCAC |
130 |
57.6 |
1.981 |
| Potri.013G082800 |
R: CGGGCAGGCATTTATCTG |
|||||
|
BSP C |
Bark Storage Protein C |
POPTR_0013s10350 |
F: TTCGTGGTGTTCCAAGGTG |
85 |
54 |
1.936 |
| Potri.013G101000 |
R: AGGCGTTGTAGGAGGCTAAG |
|||||
|
WIN4 |
Wound Induced 4 |
POPTR_0423s00200 |
F: AGGATTTTCGCCTGCTGG |
64 |
62.6 |
2.032 |
| Potri.013G080500 and/or Potri.013G082800 |
R: AATGAACTTGGCTGCGGC |
|||||
|
PNI 288 |
Poplar nitrogen-regulated cDNA 288 |
POPTR_0019s07690 |
F: TGCCAATAGATTCAATGCCAC |
60 |
55 |
1.976 |
| Potri.019G050200 |
R: GAAGCCAAAGCAACAGCAG |
|||||
|
VSP 87A |
WIN-like VSP 87A |
POPTR_0013s07850 |
F: TGAACGGAGAGAACTTGTTGGC R: AGGATGTGGTGCTGGGAAGC |
105 |
54 |
1.912 |
| Potri.013G082600 | ||||||
|
VSP 425 |
WIN-like VSP 425 |
POPTR_0013s07810 |
F: CAAATGTAGCAGGTGAAGCAAG |
154 |
54 |
1.983 |
| Potri.013G080400 |
R: TCAAACGACTCAGAAGCAGATAC |
|||||
|
VSP XIII |
WIN-like VSP XIII |
POPTR_0013s07800 |
F: TCCAGGATTATCGCCTGCTA |
110 |
55 |
2.040 |
| Potri.013G080300 |
R: AATCCCATCACTCACAAGCC |
|||||
|
VSP 840 |
WIN-like VSP 840 |
POPTR_0013s07840 |
F: CCTCCTACAATGCTTTCCTTGCTG |
91 |
55 |
1.96 |
| Potri.013G082700 |
R: GCAGATACAAAATCCCATCACTCAC |
|||||
|
NP 157 |
NP-like 157 |
POPTR_0006s16610 |
F: GCTGTAGATGCTTCACTTAGGTTC |
65 |
54.4 |
1.986 |
| Potri.T096300 |
R: GCCTTATTCGGTAGTTCCAAC |
|||||
|
NP 860 |
NP-like 860 |
POPTR_0008s02860 |
F: TCAAACGGGTATCCTGTGATTGTC |
96 |
61 |
2.07 |
| Potri.008G028500 |
R: TGCTAAGGGTCCAAATGTCTGG |
|||||
|
NP 870 |
NP-like 870 |
POPTR_0008s02870 |
F: AGGGGATGGAACTGGAGAAGTG |
102 |
60 |
2.01 |
| Potri.008G028600 |
R: CCACGAAAATGTCTGCGGTTG |
|||||
|
NP 880 |
NP-like 880 |
POPTR_0008s02880 |
F: GCTACCAGGATACAACTCTCCATTG |
127 |
55 |
1.99 |
| Potri.008G028700 | R: GCTGAAGAACCCCTAAAGATGTCTC |