Fig. 2.

Within the general reaction scheme, the changes in the amounts of three prominent reaction species are detected to determine if a stabilizing ligand/corrector of the fold is present. (1) The aggregation based screening system relies on the appearance or disappearance of downstream aggregates. (2) The fluorescence thermal scanning systems rely on the appearance of partially folded forms of the protein to determine when a potential ligand/corrector is present. (3) The chaperonin based capture system discussed in this review detects the appearance or decrease in the initial transient fold, giving this assay the advantage of identifying potential stabilizing ligands/correctors under near physiological solution conditions.