Fig. 4.

Using a similar setup reported by Murai et al., Surface Plasmon resonance technologies was used to determine if the immobilized form of protein alpha lactalbumin has acquired it’s proper fold compared to its partially folded form (disulfide reduced form) by comparing the extent of chaperonin binding to each species. When the protein has acquired its proper fold, there is a dramatic signal decline in SPR response units as chaperoninbinding decreases.