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ICP0 does not undergo proteasome-dependent degradation. HEL fibroblasts infected with 10 pfu of HSV-1(F) (lanes 2–7) or R7914 (lanes 9–14) per cell were mock-treated or treated with MG132 for 1 h before harvest at indicated times. The lysates were solubilized and subjected to electrophoresis in denaturing gels and reacted with a mouse monoclonal antibody against ICP0.
