FIG. 1.
Lymphocyte proliferation assay. BALB/c mice were immunized with pcDNA-SOD and the parental plasmid pcDNA3. The T-cell proliferative response was measured at week 4 after immunization. Splenocytes from each group (4 × 105 per well) were prepared and stimulated in vitro with CBPs (4 μg/ml) and purified rSOD (0.4 μg/ml) as antigens. Each value is the average number of counts per minute of triplicate cultures of cells (± standard deviation) obtained from a pool of five mice in each group. *, P < 0.005 compared with the value for pcDNA3-immunized control mice.