FIG. 4.

Lymphocyte proliferation assay for T-cell populations. BALB/c mice were immunized with pcDNA-SOD (solid bars) or the parental plasmid pcDNA3 (open bars). At week 4 after immunization, splenocytes from each mouse were separated by flow cytometry into CD4⁺ (A) and CD8⁺ (B) T cells and stimulated in vitro with CBPs (4 μg/ml) and purified rSOD (0.4 μg/ml) as antigens. Each value is the average number of counts per minute of triplicate cultures of cells plus standard deviation (error bars) obtained from a pool of five mice in each group. *, P ≤ 0.05 compared with the value for c-RPMI medium.