Table 1.
Mutation | Sequence of the primers | mp | Product length |
---|---|---|---|
5382insC |
N: 5’ AGAGAATCCCAGGACA 3’ |
68,2°C |
168 pz |
M: 5’ AGAGAACTCCCAGGAC 3’ |
81,8°C |
||
R: 5’ ATATGACGTGTCTGCTCCAC 3’ |
64,1°C |
||
185delAG |
N: 5’ GCTGACTTACCAGATGGGACTCTC 3’ |
66,1°C |
335 pz |
M: 5’ CCCAAATTAATACACTCTTGTCGTGACTTACCAGATGGGACAGTA 3’ |
78,4°C |
||
R: 5’ GGT TGG CAG CAA TAT GTG AA 3’ | 63,4°C |