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. 2013 Aug 13;11(1):10. doi: 10.1186/1897-4287-11-10

Table 1.

The sequences of the primers used in the ASA-PCR reaction

Mutation Sequence of the primers mp Product length
5382insC
N: 5’ AGAGAATCCCAGGACA 3’
68,2°C
168 pz
M: 5’ AGAGAACTCCCAGGAC 3’
81,8°C
R: 5’ ATATGACGTGTCTGCTCCAC 3’
64,1°C
185delAG
N: 5’ GCTGACTTACCAGATGGGACTCTC 3’
66,1°C
335 pz
M: 5’ CCCAAATTAATACACTCTTGTCGTGACTTACCAGATGGGACAGTA 3’
78,4°C
  R: 5’ GGT TGG CAG CAA TAT GTG AA 3’ 63,4°C